The experiment was carried out at the Teaching and Research farm of Joseph Sarwuan Tarka University Makurdi, Makurdi (6° 11’-7° 41’N Latitude and 7° 21’ - 8° 37’E Longitude). The experiment aimed to investigate the effect of effects of organic manure on the nutritional composition of rosselle seed, leaves and calyx in Makurdi, Benue state Nigeria. The experiment was done in the laboratory of Animal Production Laboratory of Joseph Sarwuan Tarka University Makurdi. The treatments used were Seeds, Leaves and Calyx of rosselle from NG001(Dark red), NG002 (Wine red) and NG003 (Light red) accession that was grown using organic nutrient source of Poultry droppings, Cow dung, Compost and Control, the nutritional components that was determined from the seeds, leaves and calyx were moisture, crude fat, ash, crude fiber, crude protein and carbohydrate through the following methods; Moisture Content Oven Drying Method was used, Crude Fiber Weende Method, Lipids Soxhlet Extraction, Ash Dry Ashing, Protein Kjeldahl Method and Carbohydrate Phenol-sulfuric acid was used. From the result obtained significant difference (P<0.05) was recorded were NG001 (Dark red) accessions had higher nutritional content when compared with other accession used with NG003 (light red) recording the least. On the nutritional content the Calyx recorded the highest in carbohydrate (60.21) content with Leaves recorded the highest in protein content (11.26), moisture content (10.74), crude fat (8.21), ash (7.24) and crude fibre (6.24) while Seed recorded the least in all the nutritional content in NG001. From the result obtained farmers in these location are advice to used to use poultry dropping in cultivating roseelle as it add to the nutritional content of the plants thereby improving human health.