This study investigates the challenges and solutions associated with implementing Life Cycle Costing (LCC) for the procurement of medical laboratory equipment in selected federal healthcare institutions in Enugu State, Nigeria. Despite widespread awareness of LCC among procurement officers, biomedical engineers, and laboratory scientists, its practical application remains inconsistent across procurement stages. The study employed a mixed-methods research design, combining quantitative data from 273 usable questionnaires and qualitative insights from semi-structured interviews and focus group discussions. Findings reveal that while respondents possess knowledge of key LCC methods, including Internal Rate of Return (IRR) and Equivalent Annual Cost (EAC), and are aware of major cost parameters such as initial investment, operational, and maintenance costs, the actual integration of LCC into planning, implementation, and evaluation is limited. Significant challenges hindering effective LCC adoption include inadequate institutional guidelines, insufficient training, insufficient data quality, and the absence of appropriate software tools. The study further identifies the benefits of LCC in improving cost forecasting, risk assessment, scenario analysis, and profitability, highlighting its potential for sustainable procurement practices. To bridge the gap between awareness and practice, the study recommends capacitybuilding initiatives, the development of standardised LCC frameworks, the investment in software tools, and the integration of LCC into institutional procurement policies.